Standard curve: (1) Linear from 0.05 to 25 ng/mL; LLOQ: 0.05 ng/mL; r2 > 0.999 (for 5500 QTRAP system). (2) Linear from 0.5 to 25 ng/mL; LLOQ: 0.5 ng/mL; r2 > 0.990 (for Exactive Orbitrap system). Accuracy and precision: (1) percent Bias typically between: -8.0 to 8.0. (2) percent CV typically between: 1.2 to 5.8. Spotted blood volume, punch location and punch size: (1) No significant differences observed. Recovery: (1) Extraction recovery and overall method recovery averaged 80percent for both guanfacine and its IS. Long-term stability QC samples: (1) DBS QC samples analyzed after 33 days and 76 days showed no significant deviation regarding accuracy and precision compared to freshly prepared DBS QC samples.
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机译：标准曲线：（1）线性为0.05至25 ng / ml; lloq：0.05 ng / ml; R2> 0.999（适用于5500 Qtrap系统）。 （2）从0.5至25 ng / ml的线性; lloq：0.5 ng / ml; R2> 0.990（用于辐射锻造系统）。准确性和精确度：（1）偏差百分比之间：-8.0至8.0。 （2）CV百分比，通常之间：1.2至5.8。斑点血容量，打孔位置和冲压尺寸：（1）未观察到显着差异。恢复：（1）提取恢复和整体方法恢复平均为关菲岛及其是的80％。长期稳定性QC样品：（1）与新制备的DBS QC样品相比，33天和76天分析的DBS QC样品显示无明显和精度的显着偏差。
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