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Proteomic Analysis of Lipid Rafts of Abca1~(-/-) Macrophages Reveals Novel Signaling in Lipid Rafts

机译:ABCA1〜( - / - )巨噬细胞脂质筏的蛋白质组学分析揭示了脂筏中的新型信号

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A semi-quantitative spectral counting approach was evaluated to study expression changes in lipid raft proteins of Abcal~(+/+) and Abcal~(-/-) bone marrow macrophages after LPS exposure. LC-MS/MS data indicate that several proteins exclusively recruited/highly expressed in lipid rafts of Abcal~(-/-) macrophages. Few protein showed significant fold changes due to knockout and LPS exposures. Further studies are underway to validate their functional roles in lipid rafts.
机译:评估了半定量光谱计数方法,以在LPS暴露后的Abcal〜(+ / +)和ABCAL〜( - / - )骨髓癌中的脂筏蛋白中的表达变化。 LC-MS / MS数据表明,在ABCAL〜(/ - / - )巨噬细胞的脂质筏中专门募集/高度表达的几种蛋白质。很少有蛋白质显示出由于敲除和LPS暴露而显着的折叠变化。正在进行研究以验证脂质筏中的功能作用。

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