Acyl-coenzyme A Profile Study in Tissues of Wild Type and Short-Chain hydroxyacyl-CoA Dehydrogenase (SCHAD) Knockout Mice by Tandem Mass Spectrometetry

摘要

Current published methods only provide limited quantitation for several acyl-CoAs of either long- or medium chain lengths. Lack of complete information of the profile of different chain length acyl-CoA species makes it difficult to have a clear view of the roles acyl-CoAs play in FAO defects. Our approach successfully identifies the unique fragmentation pattern that all acyl-CoA species have. There is a characteristic fragment resulting from a loss of m/z 507 within the collision cell. This observation allowed us to establish an assay based on this neutral loss scan to obtain a complete acyl-CoA profile of a set of acyl-CoA standards and murine tissue extracts. Comparison of acylcarnitine data from the same tissues indicate that acylcarnitine and acyl-CoA's do not always correlate. Analysis of acyl-CoA profiles provides us with a new tool for the in-depth study of the roles of all acyl-CoA species in FAO metabolism and in other metabolic pathways.