Utilization of GCXGC-TOFMS to Screen for Potential Metabolite Differences in Pooled Samples from Lean, Fat, and Obese Zucker Rat Plasma

摘要

This study highlights the use of GCxGC-TOFMS analysis of pooled Zucker rat plasma samples from different states of health. The chromatographic and time-of-flight mass spectral comparisons between sample pools establishes this analytical approach as a valuable screening tool for discovery of metabolite variation across different classes. The analysis of derivatized complex blood plasma demonstrates the enhanced detection and increased peak capacity available with GCxGC-TOFMS using the LECO Pegasus 4D instrument. The GCxGC-TOFMS results for the lean, fat, and obese sample pools demonstrates how GCxGC separates and resolves components that would otherwise totally coelute and be buried under high concentration analytes in one-dimensional chromatography. TOFMS provides non-skewed mass spectral data and the fast acquisition necessary to facilitate deconvolution algorithms that yield high quality library searchable mass spectra. This proof of concept research shows a fast, efficient, and simple comparative analysis method for screening large sample pools to distinguish metabolite variations as potential biomarkers for healthy and disease state populations. GCxGC-TOFMS is a valuable time and resource savings instrumental option for the comparative analysis of pooled samples from varying states of health to quickly and efficiently determine significant metabolic differences.