Secretion Vesicle Proteomics: Glioblastoma Exosome and Modulation by Wild-Type p53 Genetherapy

摘要

Triplicate analysis of exosomes harvested from U87 and U251 after treatment with wt-p53 and empty vector provide relative quantitation of proteins enriched in cell-to-cell interactions, cell death, and cancer biology. Exosomes are believed to be involved with cell-to-cell interactions through the "bystander" effect. Enrichment of proteins associated with cell-to-cell interactions are reported by our proteomic results and previous work by other laboratories. Treatment of glioblastoma with wt-p53 is known to induce apoptosis. Several proteins were identified to be differently regulated between the wt-p53 treated and empty-vector treated cells. Furthermore, differences in protein regulation are observed between U87 and U251 glioblastoma cell lines.