Quantitative proteome analysis of pluripotent cells by iTRAQ mass tagging identifies post-transcriptional regulation of proteins required for ES cell self-renewal

摘要

iTRAQ labeling is amenable to comparison with microarray data, since both use comparators but confirmation experiments are essential. Pluripotent cells switch from glycolysis to aerobic metabolism as they differentiate: this switch is a major marker of differentiation. Pluripotent cells maintain high levels of rRNA synthesis proteins before differentiation: major gene ontology enriched in ES and EC cells. Pdlim7 is post-transcriptionally regulated in EC cells: stabilized in undifferentiated cells/destabilized in differentiated cells. Racgap1 is post-transcriptionally regulated in ES cells and is necessary for ES self-renewal.