Determination of cancer biomarkers using targeted MS analysis of isotope isotopelabeled formalin formalin-fixed paraffin paraffin-embedded tissue samples

摘要

Successful detection of these proteins from FFPE samples proves that mass spectrometric analysis of such samples is feasible and that we can now avail of banked samples for potential marker validation. In addition since these kinds of samples have been collected for many years, in many cases patient outcome is already known, thus the approach also provides an ideal means of assessing the prognostic value of potential markers. Lastly, on-going experiments designed to determine the absolute abundance of some of these markers are progressing. The only difference from the experiments conducted above would be the labeling of known amounts of synthetic peptides from the markers of interest in place of the reference sample. We have recently demonstrated the feasibility of this aspect using tissue homogenates and will be extending the approach to these samples as well.