From Biopsy to Cartilage-carrier-constructs by Using Microcarrier Cultures as Sub-process

摘要

The production of cartilage-implants includes several steps of propagation accopamied by an unwanted dedifferentiation of the cells. The aim of this work was to investigate the propagation of human chondrocytes on microcarriers in a specially designedconical bioreactor as a sub-process for the production of cartilage-carrier-constructs. This technique for increasing the number of chondrocytes without losing their characteristic phenotype and ability to produce cartilage matrix can improve the development and quality of hyaline-like cartilage implants.