SINGLE SHOT DIA PROFILING OF >1500 PLASMA PROTEOMES OF THE WEIGHT LOSS AND MAINTENANCE STUDY DIOGENES

摘要

Comprehensive, robust, high-throughput analysis of the plasma proteome has the potential to enable analysis of the health state. Up to now, analysis of the plasma proteome was achieved using delicate nanoflow setups. To reduce this limitation, we established a robust capillary-flow LC-MS-DIA setup capable of measuring 32 plasma proteomes a day. Using this setup, we acquired the samples of a large-scale weight loss and maintenance plasma sample study (DiOGenes).