APPLICATION OF TRIPLE QUADRUPOLE MS FOR DRUG-TO-ANTIBODY RATIO AND MOLECULAR WEIGHT DETERMINATION OF ANTIBODY-DRUG CONJUGATES

摘要

Antibody-drug conjugates (ADCs) are an increasingly popular class of therapeutic drugs with an inherently high heterogeneity from a large arsenal of drugs, linkers and antibodies. RPLC-MS using QTOF or Orbitrap analyzers have been used successfully to determine the average drug-to-antibody ratio (DAR) for several constructs. Here we have investigated the potential for the very common triple quadrupole (QqQ) analyzers as an alternative for this application.