Qualitative workflows have employed nanoflow LC-MS for sample-limited analysis of complex mixtures. The intrinsic complexity of these samples requires long, slow gradients to maximize separation capacity. Quantitative applications requiring higher throughput are traditionally run at conventional LC flow rates (100μL/min. and higher), limiting the ability to analyze small sample volumes. High-performance microspray, defined here as flow rates of 1 - 10μL/min. delivers the much of the sensitivity realized by nanoflow LC-MS while enabling efficient duty cycle and throughput. Nanospray source hardware has traditionally been used at flows ranging from 20nl/min. to 1000nl/min. We investigate the capability of a modified nanospray source, using conventional nanospray emitters and columns to operate at 1μL/min. to 10μL/min. by evaluating spray stability and analyte response.
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