Pseudomonas aeruginosa develops Ciprofloxacin resistance from low to high level with distinctive proteome changes

摘要

Pseudomonas aeruginosa PAO1 develops Ciprofloxacin resistance from low level to high level. Different concentrations of Ciprofloxacin selected different resistant mutants. Anaerobic respiration and catalases contributed to the low level resistance in mu0125_l. Ion, polyamine and DNA repair mainly contributed the low level resistance in mu05_l. High level resistances inevitably involved the efflux pump mexCD-oprJ, although other protein networks were involved in the resistance. Intracellular Ciprofloxacin concentration was not affected in the low level resistant mutants but is kept low at the very beginning by efflux pump mexCD-oprJ in the high level resistant mutants.