Probing protein surface chemistry and unfolding pathways by coupling gas-phase HDX and native MS

机译：通过耦合气相HDX和天然MS探测蛋白质化学和展开途径

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- A modified Synapt G1 enabled gas-phase HDX on a protein surface. - Gas-phase HDX successfully discriminates between different protein surface residue gas-phase basicities. - Use of GFP and its surface mutants show the potential importance of carboxylic acid groups in surface exchange. - Coupling gas-phase HDX and ion mobility separation can allow for conformer specific labelling. - Each conformer displays uptake specific to its gas-phase structure. - Offers potential for gaining structural insights into CIU intermediate.

机译：- 在蛋白质表面上启用了改进的Synapt G1使能的气相HDX。 - 气相HDX成功鉴别不同的蛋白质表面残留气相碱基。 - GFP及其表面突变体的使用表明羧酸基团在表面交换中的潜在重要性。 - 偶联气相HDX和离子迁移率分离可以允许符合特征的标记。 - 每个构象器显示出特异于其气相结构的摄取。 - 提供对Ciu中间体的结构见解的潜力。

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《ASMS Conference on Mass Spectrometry and Allied Topics》|2016年|1CD|共1页
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Shane A. Chandler; Todd H. Mize; Ulrik H. Mistarz; Jeffery M. Brown; Kasper D. Rand; Mike R. Morris; Justin L. P. Benesch;
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1. Monitoring of the functional properties and unfolding change of Ovalbumin after DHPM treatment by HDX and FTICR MS Functionality and unfolding of Oval after DHPM by HDX and FTICR MS [J] . Liu Guang-xian, Tu Zong-cai, Wang Hui, Food Chemistry . 2017,第JULa15期

机译：通过HDX和FTICR MS监测DHPM处理后卵清蛋白的功能特性和展开变化通过HDX和FTICR MS监测DHPM处理后卵清蛋白的功能变化和椭圆
2. Installation, validation, and application examples of two instrumental setups for gas-phase HDX-MS analysis of peptides and proteins [J] . Ulrik H. Mistarz, Kasper D. Rand Methods: A Companion to Methods in Enzymology . 2018,第期

机译：用于肽和蛋白质的气相HDX-MS分析的两种仪器设置的安装，验证和应用示例
3. Protein Conformations Can Be Probed in Top-Down HDX MS Experiments Utilizing Electron Transfer Dissociation of Protein Ions Without Hydrogen Scrambling [J] . Abzalimov RR, Kaplan DA, Easterling ML, Journal of the American Society for Mass Spectrometry . 2009,第8期

机译：可以在自上而下的HDX MS实验中探索蛋白质构象，该实验利用蛋白质离子的电子转移解离而不加氢
4. Probing protein surface chemistry and unfolding pathways by coupling gas-phase HDX and native MS [C] . Shane A. Chandler, Todd H. Mize, Ulrik H. Mistarz, ASMS Conference on Mass Spectrometry and Allied Topics . 2016

机译：通过耦合气相HDX和天然MS探测蛋白质化学和展开途径
5. Tools for probing protein higher-order structure: Monitoring aggregation with native ESI-MS and conformational dynamics with top-down h/d exchange MS. [D] . Wang, Guanbo. 2013

机译：探测蛋白质高阶结构的工具：使用天然ESI-MS监测聚集，并使用自上而下的h / d交换MS监测构象动力学。
6. Protein conformations can be probed in top-down HDX MS experiments utilizing electron transfer dissociation of protein ions without hydrogen scrambling [O] . Rinat R. Abzalimov, Desmond A. Kaplan, Michael L. Easterling, -1

机译：蛋白质构象可以在利用蛋白质离子的电子转移解离没有氢扰自上而下HDX ms实验被探测
7. Protein Conformations Can Be Probed in Top-Down HDX MS Experiments Utilizing Electron Transfer Dissociation of Protein Ions Without Hydrogen Scrambling [O] . Abzalimov Rinat R., Kaplan Desmond A., Easterling Michael L., 2009

机译：可以在自上而下的HDX MS实验中探索蛋白质构象，该实验利用蛋白质离子的电子转移解离而不加氢

Probing protein surface chemistry and unfolding pathways by coupling gas-phase HDX and native MS

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