The ETD-MS/MS data showed an abundant cleavage after amino acid 44. The motif being clipped is YAERVG-AGAPV. After this cleavage, a 9228.20502 Da N-terminal polypeptide is generated, and opposite to the intact protein, the abundance of this peptide increases over time. Analysis of the digested samples by SDS gel confirmed that Cathepsin L is able to digest H2A in-vitro. After one hour, the amount of intact histone drastically decreased compared to the input.
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