Trans-omics Analysis of Yeast Central Carbon Metabolism by Integration of Metabolome, Proteome and Fluxome Data

摘要

The central carbon metabolism is an old frontier of biology, but recently revisited with the mass spectrometry-based trans-omics analysis to investigate the cancer-specific metabolism and a microbial cell factory producing biomaterials. Although the central metabolic pathways, intermediates, and enzymes in an eukaryotic cell model, Saccharomyces cerevisiae, have been well characterized, a system-level understanding of the regulation network requires a quantitative description of those complex behaviors. Recent progress in mass spectrometry-based bioanalysis techniques enabled to produce the required amount of quantitative data from S. cerevisiae. In this study, a trans-omics dataset including metabolome, proteome and fluxome data was obtained from the 11 single-deletion strains of S. cerevisiae by the ion-pairing liquid chromatography-mass spectrometry (LC-MS), the targeted proteome analysis using the fast scanning speed of LC-ultra-fast mass spectrometry (LC-UFMS), and the ~(13)C-metabolic flux analysis (MFA) using GC-MS.