Accelerating 2D FT-ICR by nonuniform sampling (NUS) and maximum entropy reconstruction for increased resolution and/or decreased acquisition time

摘要

Successful implementation of 2D FTICR-MS on a unmodified commercial instrument using gas-free fragmentation modes (IRMPD, ECD) which preserve FT-ICR resolution. New pulse design which decreases the scintillation noise and optimize sample efficiency. Reduction of the scintillation noise using dedicated robust algorithms (urQRd) which can be applied to 1D spectrum also. Achieved improved FT-ICR resolution in the horizontal dimension (MS and MS/MS spectrum) and improved quadrupole-like resolution in the vertical dimension (precursor ion identification) using NUS or acquisition on first points and the newly designed algorithm RECITAL. A true "Data Independent Acquisition" showing all the correlation of a sample (fragment, parent, neutral loss, charge gain or loss).