Targeted quantitative proteomics of protein biomarkers for plasma nutrient status using heavy stable isotope labeled recombinant proteins

摘要

Heavy stable isotope labeled internal standard proteins are advantageous for absolute quantitation. Targeted selected reaction monitoring and identification of proteins can be performed in a single assay, shown here with plasma RBP4, VitD-BP, ApoAI and TTR. argeted quantitation data strongly correlated with relative abundance data (iTRAQ). Plasma RBP4 and TTR correlated with vitamin A and Apo-AI correlated with plasma levels of vitamin E, as predicted from relative abundance analysis. Vitamin D and its quantitated binding protein did not, a difference that is currently under study.