Mass Spectrometric Quantification of Cardiolipin in Human Serum by MALDI-TOF with HPLC/ESI-MS Validation

摘要

Cardiolipin internal standard has been successfully incorporated into BSA solutions and extracted with >95percent efficiency and isolated from other phospholipid classes Detection limits of either MALDI or LC/ESI MS were inadequate to measure endogenous cardiolipin in human serum [100fmol/(mu)l] Currently, the application of the methodology using LCQ Deca XP plus has lowered the detection limit to fmol/(mu)l, with a lower detection limit of 25 fmol/(mu)l