UPLC-MS/MS Determination of Cortisol and 6beta-Hydroxylcortisol in Human Urine -Comparison with HPLC-MS/MS method

机译：UPLC-MS / MS测定人尿液中皮质醇和6β-羟基醇的测定 - 用HPLC-MS / MS法比较

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An ULPC-MS/MS method was developed for quantitative determination of E and 6beta-OHE in human ruine in the range of 1-150 ng/mL and 5 - 75 ng/mL for E and 6beta-OHE, respectively. The assay performance (accuracy and precision) is similar for UPLC-MS/MS method and HPLC-MS/MS method. The UPLC-MS/MS method significantly reduces the run time with better separation efficienty, higher sensitivity, and reduced matrix effects comparing to the HPLC-MS/MS method.

机译：开发了ULPC-MS / MS方法，用于分别为1-150ng / ml和5-75ng / ml的人类毒物中的E和6β-OHE的定量测定，分别为E和6β-OHE。测定性能（精度和精度）类似于UPLC-MS / MS方法和HPLC-MS / MS方法。 UPLC-MS / MS方法显着降低了与HPLC-MS / MS方法比较的更好分离，更高的分离，更高的敏感性和降低的矩阵效应。

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《ASMS Conference on Mass Spectrometry and Allied Topics》|2008年||共1页
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Yuwen Zhao; Lina Tang; Jamie Zhao; Yuan-Shek Chen; Yongdong Zhu; Kumar Ramu; Benjamin Chien; Ken Blakeslee;
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中图分类 TB994.5-53;
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UPLC-MS/MS Determination of Cortisol and 6beta-Hydroxylcortisol in Human Urine -Comparison with HPLC-MS/MS method

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