Automatic off-line enrichment of phosphorylated peptides by titanium dioxide

摘要

Development of an automatic off-line method for the enrichment of p-peptides Requirements: Compatible with in-gel and in-solution digests. Unattended processing of samples from micro titer plates. Collection of bound and unbound fraction for further analysis. Compatible with labeling techniques for protein quantitation such as iTRAQ. Tolerant with salt to be compatible with IEX-chromatography