Supercritical carbon dioxide extraction (SCCO_2) of Chiba seed (Psoralea corylifolia) and LC-MS characterization of the of the extract

摘要

The SC CO_2 extracts of Chiba Seeds (Psoralea corylifolia) are obtained at pressures (18, 28 and 38 MPa) and at three temperature (40°,50° and 60°C) to obtain extracts rich in bakuchiol to optimize the conditions of extraction. The extracts are analyzed for bioactive components bakuchiol, psorlene isopsoralene. Bakuchiol is the main component of interest is obtained by SC CO_2 extraction in substantial quantities (>50%) and the extraction by traditional solvent extraction results in poor recovery as it was found to be thermally labile. Supercritical fluid carbon dioxide extraction (SCFE CO_2), a novel method for extraction, more relevant for obtaining thermally labile natural products, is cost effective and eliminates toxic organic waste. The SC-CO_2 extracts were investigated for the occurrence of different components bakuchiol, psoralen, isopsoralen, corylin, psoralidin, bavachromene, isobavachalcone, corylifol A, bavachlcone, Brosimachutin G, bakuchiol and was confirmed by high-performance liquid chromatography (HPLC) and by Liquid chromatography - Mass Spectrometry (LC-MS) in electron spray ionization (ESI) negative mode. The structures of bakuchiol, sporalen and isosporalen are confirmed by 500 MHz NMR studies. Most of the compounds has reported to have high degree of antibacterial activities and anticancer properties.