Bile Nucleotides Exaggerate Ischemia-Reperfusion-lnclucecl Epithelial Injury via P2Y, Not P2X Purinoceptor in Rat Jejunum

摘要

Extracellular nucleotides have important roles to mediate and regulate the physiological functions such as neurotransmission and secretion [1,2]. Bile contains enough concentration of nucleotides to activate purinoceptors [3], such as P2X and P2Y adenosine triphosphate (ATP) receptor expressed in the lumen of epithelial cells [2]. However, the effects of luminal nucleotides on the intestinal epithelial functions are unknown. We have demonstrated that luminal taurocholic acid (TCA) acidifies epithelial cells and enhances ischemia-reperfusion (I/R)-induced epithelial injury in rat jejunum [4]. The present study was undertaken to examine the effects of luminal nucleotides with or without TCA on intracellular pH (pHj) and cell injury induced by I/R in rat jejunal epithelia.