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Effects of Plant Origin Preparations on the Growth of Fungi Isolated from Seeds of Selected Vegetables

机译:植物来源制剂对从选定蔬菜种子中孤立的真菌生长的影响

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Alternaria alternata, A. brassicae, A. brassicicola. Fusarium avenaceum, F. oxysporum, Phoma lingam, Penicillium corymbifentm and P. cyclopium were isolated from cabbage seeds. A. alternata. A. dauci and A. radicina were isolated from carrot seeds andA. potri, Botrytis aclada, B. cinerea, Fusarium oxysporum and Stemphylium botryosum from onion seeds. The fungi were grown in Petri dishes on plain potato dextrose agar (PDA) as well as on the medium supplemented with Citrosept (a.i. grapefruit extract), Fitoekols-IF (a.i. pine and spruce needles extract) or standard recommended fungicides, i.e. metalaxyl, carbendazim, thiram and iprodione against fungi isolated from cabbage seeds, iprodione against fungi from carrot seeds and benomyl, metalaxyl and thiabendazole against fungi from onion seeds. Citrosept was added to PDA in three concentrations: 0.05, 0.1 and 0.5 %, whereas Fitoekols-IF was added at concentration of 2.0 %. Petri dishes were placed in darkness at 20 °C. Diameters of fungal colonies were measured after 7 and 14 days of incubation. Both plant origin preparations inhibited growth of the examined fungi, but Citrosept at a concentration of 0.5 % controlled growth of fungi to the largest extent.
机译:交链孢,甘蓝A.,A甘蓝。镰孢avenaceum,尖孢镰孢,茎点的lingam,青霉corymbifentm和P. cyclopium从卷心菜种子中分离。 A.病菌。 A. dauci和A. radicina从胡萝卜种子和A隔离。 potri,灰霉病aclada,灰葡萄孢,尖镰孢和从洋葱种子匍柄botryosum。真菌在在普通马铃薯葡萄糖琼脂(PDA)以及补充有Citrosept在介质上(AI葡萄柚提取物)的培养皿中生长,Fitoekols-IF(AI松树和云杉针叶提取物)或标准推荐的杀真菌剂,即甲霜灵,多菌灵,福美双和异菌脲对来自甘蓝种子,对来自胡萝卜种子和苯菌灵,甲霜灵和噻菌灵针对来自洋葱种子的真菌的真菌分离异菌脲真菌。 Citrosept加入到PDA在三个浓度:0.05,0.1和0.5%,而Fitoekols-IF以2.0%的浓度加入。培养皿在20℃下在黑暗中放置。后第7和14天的潜伏期,测定真菌菌落的直径。两个植物来源制剂抑制被检查真菌的生长,但在Citrosept真菌最大程度0.5%受控生长的浓度。

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