CREATION OF TRANSGENIC TOBACCO PLANTS WITH ENHANCED PHOTOSYNTHETIC CAPACITY USING CHLOROPLAST TRANSFORMATION TECHNOLOGY

摘要

We have previously generated transgenic tobacco plants which were introduced a fbp/sbp encoding fructose- l,6-/sedoheptulose-l, 7-bisphosphatase (FBP/SBPase) of Synechococcus PCC7942 with a tomato promoter and a transit peptide of rbcSBC into nucleargenome by Agrobacterium-mediated gene transfer (Miyagawa et al 2001). The photosynthetic CO: fixation of transgenic plants (TpFS) was 1.24-fold higher than that of the wild-type plants under saturating irradiance condition (25 °C, 1500 mu molm~(-2)s~(-1), 360p.p.m. CO_2). Final dry matter of the TpFS plants was 1.5-fold larger than that of the wild-type plants. These data suggest that FBPase and/or SBPase associated with the regeneration of ribulose 1,5-bisphosphate (RuBP). are one of the limiting factors participating in the regulation of the carbon flow through the Calvin cycle. This is the first report in which expression of a single plastid-targeted enzyme has been shown to improve carbon fixation and growth in transgenic plants.