Refining Retinoic Acid Stimulation of Murine Adipose-Derived Stromal Cells Accelerates In Vivo Bone Formation

摘要

Previous attempts at accelerating in vivo bone formation from implantation of mouse adipose derived stromal cells involved exposing these cells, seeded on an apatite-coated PLGA scaffold, to four continuous weeks of retinoic acid exposure. Implantation of these cells into critical-sized calvarial defects resulted in rapid healing followed by osteoclast-mediated bone resorption. This was thought to be mediated by residual retinoic acid on the scaffolds. As a follow-up study, exposure of mouse ASCs in monolayer to shorter courses of retinoic acid, interestingly, revealed accelerated os-teogenic differentiation in vitro. This study seeks to investigate whether such an approach can be translated into the in vivo setting.