Role of Soy Phytoestrogens Genistein and Daidzein in Focal Adhesion Assembly and Focal Adhesion Kinase Activity in Breast Cancer Cells

摘要

Recently, our laboratory and others have shown that estrogen receptor (ER)a. negative (-) breast cancer (BC) cells respond to estrogenic compounds via rapid signaling mechanisms. To understand the role of soy phytoestrogens in BC cell invasion, we analyzed focal complex formation and focal adhesion kinase (FAK) activity, as detected by phospho-specific anti-FAK antibodies to tyrosine residues 397 or 925. Stimulation of ERa~+ T47D and ERalpha~-^sMDA-MB-231 BC cell lines for 10 min with 17(5-estradiol (E_2), daidzein, or genistein (GEN) increased the number of focal adhesions and FAK activity. This data implicate E_2 and soy phytoestrogens in the regulation of cellular interactions with the ECM via novel signaling mechanisms.