Forensic ABO blood grouping by 4 SNPs analyses using an ABI PRISM~R 3100 genetic analyzer

摘要

This paper reports effective PCR-based methods, such as sequence-specific primers with a positive control (PCR-SSPPC) and confronting two pairs of primers (PCR-CTPP) for forensic ABO groupings using fragment analysis by ABI PRISM~R 3100 genetic analyzer. The method allows the well-established base changes at four nucleotide positions 261, 796, 802, and 803 to be assayed, so that reliable group prediction is established by the presence of three representative alleles such as A, B, and O As a result, all common alleles in the database were correctly determined by our methods.