Carrot haploid production through induced parthenogenesis

摘要

The aim of this study was to find the most efficient conditions to produce carrot haploid plants. Nineteen genetically different carrot stocks were used. Donor plants were grown in the growth chamber. For parthenogenesis induction, carrot flowers werepollinated with pollen of other species. Four induction media were used. Two years of research showed that callus and embryos were formed with the frequency of 0 to 3.87 % and 0 to 2.08 %, respectively, depending on the genotype, pollen source, and induction medium composition. 'Nantejska' produced embryos with the highest frequency (2.08 %), while 'Karlena' gave the highest frequency of callus (3.87 %). Parsley was the best pollen source in the experiment. H medium supplemented with 0,01 mg/dm~(-3) IAA was the most efficient for embryo development (1.46 %), while A12 medium supplemented with 2 mg/dm~(-3) 2,4-D and 6-BA was the most suitable for callus development (3.41 %). Plants were regenerated from callus and embryos. Ploidy level analysis of theobtained regenerants revealed the presence of diploids (98 %), no haploids were observed. Among diploids 52 % were homozygous, as proved by the Pgi isozyme test.