The application of the flow cytometry for determination of the action of serotonin on the antigenic composition and amount of DNA of plague microbe, cultured at 37°C

摘要

The results have been obtained by flow cytometry showed the significant raise of fluorescence intensity of Yersinia pestis EV cells binding FITC-labelled plague polyclonal immunoglobulins after 24 h cultivation in Hottinger broth pH 7.2 at 37°C in the presence of increasing concentration serotonin (5-Hydroxytryptamine). The presence of serotonin (5-HT) in nutrient broth at concentration of 10~(-5) M, 10~(-8) M could modulate DNA content in 37 °C growing population of plague microbe. The effect of this action depended of 5-HT concentration.