Detection of porcine reproductive and respiratory syndrome virus (PRRSV) by reverse transcriptase loop mediated isothermal amplification (RT-LAMP)

摘要

Monitoring PRRSV in boar studs is critical because of the devastating consequences of a potential PRRSV introduction and transmission to sow farms through semen. Currently, most boar studs send samples routinely (serum, blood swab and/or semen) to diagnostic laboratories to be tested for PRRSV by PCR. However, this monitoring schedule is expensive and not all boars can be tested. Moreover, the test results are usually reported the next day. The aim of this study was to explore the use of a novel molecular technique to develop a simple, rapid, and accurate on-site diagnostic test for PRRSV that could be routinely used in negative boar studs to detect PRRSV acute infection. A diagnostic test based on the recently described loop mediated isothermal amplification (LAMP) technology was developed.