The 2Fe-2S cluster in sulredoxin from the thermoacidophilic archaeon sulfolobus tokodaii strain 7,a novel water-soluble Rieske protein

摘要

The [2Fe-2S] cluster surrounding in reduced sulredoxin from the thermoacidophilic archaeon Sulfolobus tokodaii strain 7 was examined by one- and two-dimensional electron spin echo envelope modulation (ESEEM) spectroscopy. ESEEM spectra revealed two coordinated nitrogens assigned to two histidine ligands and the lines from several non-exchangeable protons. No strongly coupled protons involved in hydrogen bonds near the reduced Rieske center were detected. These results are discussed in light of the structural, redox and spectroscopic characteristics of this ubiquitous electron transfer protein family.