Measurement of Sperm Concentration in Stallion Ejaculates Using Photometric or Direct Sperm Enumeration Techniques

摘要

A highly significant correlation was detected among photometric and direct sperm enumeration techniques for evaluating sperm concentration. Depending on the instrument used, the photometric techniques tended to overestimate or underestimate sperm concentration when compared to a hemacytometer standard. Precautions should be taken when using techniques other than a hema-cytometer to measure sperm concentration, especially for stallions with large mare books where the ejaculate will be split among multiple mares for insemination.