Relationship between heterosis in F_1 hybrids and genetic similarity among parents as measured by RAPD, SSR, and co-ancestry analysis in japonica rice

摘要

The relationship between genetic similarity (GS) and performance/heterosis in F_1 hybrids among 10 japonica varieties was investigated. A diallel set of 45 crosses among 10 varieties was made. The 45 F_1s and their 10 parents were evaluated in replicated trials in the field. GS among parents was estimated using 85 simple sequence repeat (SSR) markers, 129 random amplified polymorphic DNA (RAPD) primers, and co-ancestry analysis. There were significant positive correlations among genetic similaritieson three measures. Correlations between GS and yield heterosis were not consistent among three GS measures. SSR-based GS was found to be the most applicable to predict yield heterosis or performance in the F_r However, GSs by 13 selected RAPD primers showed much higher correlation coefficients than GSs by a total of 129 primers and by SSR markers. This suggested that bands amplified with 13 selected RAPD primers should be associated with loci conferring heterosis or performance in the F_1. The relativeefficiency of three GS measures for prediction of yield heterosis in the F_1 is also. discussed in this paper.