Background/Objectives. Chlorinated solvents are common groundwater contaminants and are of significant concern for public health and the environment, requiring the development of effective remediation techniques and monitoring tools. Compound specific isotope analysis (CSIA) can be used to identify and quantify the biodegradation of chlorinated organic compounds using isotopic enrichment factors. Most lab-based isotope fractionation studies examine exposure to a single contaminant, while field sites often present a complex mixture of contaminants with inhibitory effects on biodegradation. Understanding how isotope fractionation is affected by multiple comingled chlorinated organic compounds is an essential development for the use of CSIA in field studies.
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