In this study, different micropatterns of a biocompatible hydrogel (polyethylene glycol dimethacrylate, PEGDA) were fabricated using cylindrical and spherical micro-lens-arrays (MLA) in a two-step procedure on glass substrates with reactive poly(ethene-alt-maleic anhydride) (PEMA) layers. The pattern of the protein-repellent PEGDA was produced by cross-linking using laser irradiation at 355nm through the MLAs with a periodicity of 150 or 300μm. By translating the sample horizontally or rotating it at a specific angle, and repeated irradiation, either smaller arrays or cross-like structures could be fabricated. Adsorption experiments with model proteins, albumin and fibronectin on the patterned surfaces showed that this two step procedure is suitable to produce surfaces with patterned bio-adhesive properties, which can be used to guide adhesion of living cells in a locally constraint manner.
展开▼
