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Extraction and Evaluation on Anti-fatigue Function of Polysaccharide from Auricularia auricula

机译:青贮尿嘧啶多糖抗疲劳函数的提取与评价

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The best solution to the polysaccharide ultrasonic extraction of auricularia is as follows. With aqueous conditions of solid-liquid ratio of 1:60, 4h extraction time, 40w ultrasonic power, 70°C extraction temperature, extract twice to measure the auricularia polysaccharide extraction rate as 14.95%. Then elute the auricularia polysaccharide with DEAE-Cellulose 52 chromatographic column to get auricularia polysaccharide component AAP -1 which is then separated by Sephadex G-200 gel chromatographic column to get mono component. Set up the swimming training model with increasing intensity. Feed the mouse witsh AAP-1 solution. The result shows that a certain dose of AAP-1 taken can increase the contents of muscle glycogen and liver glycogen and maintain a constant blood sugar. The exhaustion time of the mouse can be elongated and the survival time in anoxic condition will be significantly prolonged. The above shows that auricularia polysaccharide has the function of anti-exercise fatigue.
机译:多糖超声波萃取的最佳解决方案如下。含有1:60,4h提取时间,40W超声波功率,70°C提取温度,提取两次以测量Auricularia多糖提取率为14.95%。然后用DEAE-纤维素52色谱柱洗脱Auricularia多糖,得到AAP-1,然后通过Sephadex G-200凝胶色谱柱分离,得到单体组分。随着强度的增加,设置游泳训练模型。馈送鼠标Witsh AAP-1解决方案。结果表明,采取的某种剂量的AAP-1可以增加肌肉糖原和肝糖原的含量并保持恒定的血糖。鼠标的耗尽时间可以伸长,缺氧条件的存活时间将显着延长。以上表明菌丝多糖具有反运动疲劳的功能。

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