Astragalus membranaceus,seed of cowheb alters milk protein yields and lactoferrin,casein gene expression in cow Mammary Epithelial Cells

摘要

Several studies (Zhou,2005,Wu.,2004) have examined the effects of Chinese herbal medicines on milk output and quality of lactating cows in v ivo.But there were few studies reported about their undergoing mechanism in v ivo and using in v itro system to determine if medical herbs could alter milk yields and milk proteins synthesis by altering cell number and/or milk production per cell.So the present research focuses on the direct cellular level effect of aqueous extracts of A stragalus membranaceus (AM),seed o f cow heb (DSC) and the commixture of DSC and AM(DSC ＋ AM) on the cow mammary epithelial cells (MEC) cultured in v itro.The purpose of this study was to examine the alteration of α‐casein (α‐CN),β‐casein (β‐CN) and lactoferrin (LF) gene expression and milk protein secretion in response to aqueous extracts of herbs modification in cow MEC in culture.