Linking Raman-Based Phenotypic Profiling and Phylogenetic Diversity to Reveal EBPR Physiological Characteristics

摘要

Linking Raman-based phenotypic profiling and 16S-rRNA gene-based phylogenetic analysis could provide insights into SRT-dependent variations in EBPR ecology, physiology and P removal performance. This study demonstrated that multivariate analysis (e.g. HCA) of single-cell Raman spectral fingerprints can be a powerful tool for phenotypic characterization and classification of microbial ecosystems such as EBPR. Combined and simultaneous phylogenetic and phenotypic evaluation of EBPR ecosystems revealed that EBPR performance stability could be more associated with PAO phylogenetic and phenotypic diversity, than with the total PAO population abundance. The phenotypic diversity and plasticity of PAO populations, which otherwise could not be obtained with phylogenetic analysis alone, showed complex but potentially crucial association with EBPR process stability. The combination of phenotypic and phylogenetic characterization of microbial community therefore provides more insight into the potential association between EBPR phylogeny, physiological features, and EBPR performance and stability, and sheds light on the links between microbial ecology and ecosystem function/performance prediction.