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摘要

It is known that the cancer cells rapidly proliferate, as the epidermal growth factor receptor (EGFR) is overexpressed on the cell surface. EGFR is a tyrosine kinase receptor and by binding the epidermal growth factor (EGF) certain proteins are activated which will provide dimerization. Many treatment modalities and drugs have been developed to inhibit proliferation. Some of these drugs target the external region of EGFR, which is the binding site for EGF. In some cases, drug resistance is developed resulting in ineffective treatment. The aim of this study is to design a system to demonstrate physicochemical and structural differences between wild type and mutant EGFR by generating EGFRs containing mutations that provide resistance to drugs to overcome the resistance problem. This system uses EGFR mRNA as a template and expresses wild type and mutant EGFR in CHO cells to demonstrate the effect of physicochemical and structural differences on the interaction between EGF and EGFR.