Genetic Diversity Among the Microorganisms in Daqu Used for Beidacang Liquor as Revealed by RAPD Analyses

摘要

To assess the genetic diversity among five microorganisms (namely, DSL(CS)-01, DSL(CS)-02, DSL(PDA)-C, DSL(PDA)-E, DSL(PDA)-F), RAPD (random amplified polymorphic DNA) analysis was performed. Seven primers with stable polymorphisms were screened from 64 primers. PCR was carried out with the seven primers. The data indicated that the molecular markers gave different levels of polymorphism. 94 RAPD bands were obtained and out of them 64 were polymorphic bands (68.09 %) and the best primer screened was b8. The primer b8 was considered as the most effective RAPD marker for the five microorganisms above. The dendrogram built on the basis of data from RAPD analysis represented the genetic distances among the five microorganisms. Understanding the genetic variability among the microorganisms opens up a possibility for developing a molecular genetic map that will lead to the application of marker-assisted selection tools in genetic improvement of the microorganisms.