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Real-time Detection of a-Thrombin Binding to Single-Strand DNA Aptamers by a Highly Sensitive Si-Based Waveguide SPR Biosensor

机译：高灵敏度的基于Si的波导SPR生物传感器实时检测a-凝血酶与单链DNA适体的结合。

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In this paper, real-time characterization of a-thrombin binding to single-strand DNA (ssDNA) aptamers by novel Si-based waveguide SPR biosensors has been investigated. The gold nanoparticles (AuNPs) modified with anti-thrombin antibodies were employed to bind with a-thrombin via strong antibody/antigen affinity for SPR signal amplification. The detection limit of 1 pM for a-thrombin detection was achieved..

机译：在本文中，已经研究了新颖的基于Si的波导SPR生物传感器对α-凝血酶与单链DNA（ssDNA）适体结合的实时表征。用抗凝血酶抗体修饰的金纳米颗粒（AuNPs）通过对SPR信号放大的强抗体/抗原亲和力与α-凝血酶结合。 α-凝血酶检测的检测极限为1 pM。

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《Asia Pacific optical sensors conference》|2012年|p.83512R.1-83512R.5|共5页
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作者
Chi-Chieh Huang; Hsin-Feng Hsu; Sz-Hau Chen; Kun-Yu Tsai; Yang-Tung Huang; Chih-Sheng Lin; Shih-Hsin Hsu;
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中图分类 TP212.14;
关键词
SPR; surface plasmon resonance; sensor;

机译：SPR;表面等离子体共振;传感器;

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1. Highly sensitive real-time detection of DNA hybridization by using nanoporous waveguide fluorescence spectroscopy [J] . Fan Yong, Hotta Kazuhiro, Yamaguchi Akira, Applied Physics Letters . 2014,第3期

机译：纳米孔波导荧光光谱法用于DNA杂交的高灵敏度实时检测
2. Highly sensitive real-time detection of DNA hybridization by using nanoporous waveguide fluorescence spectroscopy [J] . Yong Fan, Kazuhiro Hotta, Akira Yamaguchi, Applied Physics Letters . 2014,第3期

机译：纳米孔波导荧光光谱法用于DNA杂交的高灵敏度实时检测
3. Highly sensitive sandwich-type SPR based detection of whole H5Nx viruses using a pair of aptamers [J] . Van-Thuan Nguyen, Seo Ho Bin, Kim Byoung Chan, Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices . 2016,第Null期

机译：使用一对适体，基于高敏感度三明治型SPR的整个H5Nx病毒检测
4. Real-time detection of protein kinase a activity by a Si-based ARROW-B SPR biosensor [C] . Hsu Hsin-Feng, Lin Zheng-Wen, Huang Yang-Tung, Conference on Lasers and Electro-Optics Pacific Rim . 2013

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5. The Development of an Aptamer-based Surface Plasmon Resonance (SPR) Sensor for the Real-time Detection of Glycated Protein. [D] . Zheng, Rui. 2012

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6. A Label-Free Liquid Crystal Biosensor Based on Specific DNA Aptamer Probes for Sensitive Detection of Amoxicillin Antibiotic [O] . Duy Khiem Nguyen, Chang-Hyun Jang 2021

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Real-time Detection of a-Thrombin Binding to Single-Strand DNA Aptamers by a Highly Sensitive Si-Based Waveguide SPR Biosensor

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