Preparation of Mineralized Electrospun PCL/Gelatin Scaffolds via Double Diffusion System

摘要

For successful reconstruction of skeletal defects, a range of materials including ceramics, polymers and their composites have been developed. The goal of our work is to prepare mineralized PCL/gelatin composite scaffolds in a double diffusion system as implants for bone tissue engineering application. Fibrous PCL/gelatin scaffold fabricated via electrospinning followed by immersing into disodium-β-glycerophosphate(β-GP) (10 mg/ml) for 12h were used as substrates for calcium phosphate (CaP) mineralization. The precipitation reaction was biomimetically carried out in a double diffusion system for a week. The CaP minerals precipitated on the scaffold were characterized by X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy and high-resolution transmission electron microscopy. The results show that apatite aggregates are combination of HAP, DCPD and ACP. β-GP can effectively promote the formation of CaP crystals. The composite scaffold fabricated in this paper hold promise for use in bone tissue engineering.