Comparison of Pollen Tube Growth in Style and Fruit Setting under Self-and Cross-pollination of ' Dianjiangbaiyou'Pomelo

摘要

Fruit setting percentages of ' Dianjiangbaiyou' pomelo (Citrus grandis cv.)were investigated after crossed with ' Guanximiyou' pomelo (C. grandis cv.)and ' Shatinyou' pomelo (C. grandis cv.)and the pollen tube growth in style was observed by fluorescence microscope. The results also showed the fruit setting percentage of ' Dianjiangbaiyou' pomelo was only 0.13% by selfpollination, much lower than 16.5% and 25.0% after cross-pollinated with 'Guanximiyou' pomelo and ' Shatinyu' pomelo, respectively. Selfing and crossing pollen grains on stigma germinated normally, but most of the pollen tubes of self-pollination only grew into two fifths of the upper style, and no pollen tube was observed to have reached to the style bottom, while pollen tubes pollinated by 'Guanximiyou' and 'Shatinyu' could get into the ovule and prepared for the next fertilization after 7 days. The results showed that ' Dianjiangbaiyou' pomelo was self-incompatibility.