Cell Membrane Bioeffects and DNA Trafficking Post Therapeutic Ultrasound – OptisonApplication Using Innovative Microscopy Approaches

摘要

Using direct microscopy approaches werevealed some of the bioeffects that therapeuticultrasound (TUS) alone, or with Optison, has oncells. Atomic Force Microscopy (AFM) and scanningElectron Microscopy (SEM) showed that TUS aloneaffect the cell membrane in a different mechanismcompared to TUS with the addition of Optison.Confocal-microscopy showed that TUS localizesDNA in the nucleus while Optison addition enhancesthe number of cells transfected but do not affectDNA-localization.