Patterns of yeast gene expression during fermentation and in response to stress

摘要

DNA microarrays were used to determine the genome-wide temporal patterns of gene expression during a production brewery fermentation. Among approximately 6,200 yeast genes, 1,700 were induced greater than 4-fold and 2,100 were repressed greater than 4-fold. The patterns of gene expression correlated with various landmark events of fermentation, including the initiation and cessation of cell growth, sugar consumption, and alcohol production. Interestingly, the environmental stress response was strongly induced beginning at about the time of peak fermentation activity. In order to gain a more complete understanding of this stress response, additional experiments were conducted to dissect the transcriptional response to environmental transitions normally encountered during a brewery fermentation.