DNA Chip Technology that Models Alternative Splicing

摘要

1. Hybridization conditions must be carefully optimized to enhance discrimination of signals without loss of fluorescence. 2. Results agree with Q-RT-PCR but do not reach the broad dynamic range of Q-RT-PCR. Preliminary Conclusions: Spotted arrays vs. Affymetrix 1. Results quite similar over broad range of mRNA concentrations. 2. Affymetrix more reproducible. 3. Automation of spotted array hybridizations (cDNA and oligo) will enhance reproducibility? Ron Hart et al, Rutgers University.