Antibacterial activity of Silver-Hydroxyapatite/ Titania Nanocomposite Coating on Titanium against Oral Bacteria

摘要

This study was focused on evaluating the bactericidal and anti-adhesive efficacy of silver-hydroxyapatite/ titania nanocomposites (nAg-HA/TiO_2) coating on titanium against oral bacteria. Porphyromonas gingivalis, Prevotella intermedia and Fusohacterium nucleatum and Streptococcus mutans were used. Antibacterial activity of nAg-HA/TiO_2 coating was investigated quantitatively using film applicator coating method and titanium plates incubated with bacteria were prepared for SEM to observe the adherence of oral bacteria. The viability of each type of bacteria on the antibacterial film was suppressed to about 10% after anaerobic incubation for 3 hours. Image of SEM demonstrated that bacteria on sandblasting surfaces were relatively confluent whilst on coated surfaces fewer bacteria were observed. Adherence of bacteria on nAg-HA / TiO_2-coated surfaces compared with uncoated surfaces was remarkably decreased.