Rapid molecular sensing based on on-chip size-separation process of DNA origami

摘要

Based on DNA’s affinity to a variety of functional molecules and DNA origami (DO) technology, novel DO molecules havebeen devised as molecular probes to undergo certain configuration/shape change in the presence of the target molecules. Suchreconfiguration of DO has been typically detected with other instruments such as AFM and gel electrophoresis. However, morerapid and less costly methods with improved detection limit are demanded for practical applications. In this work, aiming theestablishment of nanofluidic based detection of DO reconfiguration, the basic electrophorectic migration of various DO wascharacterized and the detection of DO reconfiguration was achieved inside a nanofluidic device called Slanted Nanofilter Array.