Surface-enhanced FTIR spectroscopy and surface plasmon resonance on biomembranes

摘要

Abstract: Lipid bilayers containing the nicotinic acetylcholine receptor were investigated by attenuated total reflection (ATR) FTIR spectroscopy and by surface plasmon resonance (SPA). FTIR-ATR spectra reveal structural details of the transmembrane protein. Surface enhanced IR absorption provides access to structural details in the close vicinity of the surface of the ATR crystal. Information about the abundance of protein substructures as well as about the orientation of those substructures are obtained. SPR was applied in order to detect changes within the adsorbed biomembrane during admission of a neurotransmitter as acetylcholine. Switching of the ion channel was successfully by SPR. This result is a step forward towards highly selective biosensors and bioactuators. !10