Infrared microspectroscopy of benign and neoplastic prostate: correlation of spectral patterns with histopathology

摘要

Abstract: The diagnosis of prostate cancer is based on the visible microscopic evaluation of both cytological and architectural features of the prostate tissue sections. In order to determine whether IR spectral 'mapping' can be used to objectively distinguish between normal and neoplastic prostate tissue, a comparison between 'visual, point-by- point' and 'automated, point-by-point' IR measurements was performed. Automated, point-by-point analysis was performed without any prior diagnostic information. Visual, point-by- point measurements were based on histopathology, histochemistry and immunohistochemical analysis of the tissue samples. The spectra obtained from these measurements were compared to the spectra obtained from automated point- by-point analysis. Our results indicate that the spectra obtained from histopathologically directed measurements compares well with those of automated mapping methods. Therefore, we believe that current mapping methodology can be directly correlated with pathological diagnoses. !18